目的研究采收期内铁皮石斛多糖类成分含量比较。方法利用苯酚-硫酸法测定多糖的含量，高效液相法测定甘露糖的含量及甘露糖与葡萄糖的峰面积比值。结果铁皮石斛多糖含量为26。52%~46。78%，甘露糖含量为13。36%~25。25%，甘露糖与葡萄糖峰面积比值为2。48~5。91。结论不同来源的铁皮石斛中多糖类成分含量有较大差异，但均符合药典标准，其中云南产的多糖类成分含量高于浙江产，2年生及以上样品甘露糖含量高于1年生。

OBJECTIVE To compare the polysaccharide contents of Dendrobium officinale in collection periods. METHODS The contents of polysaccharide and mannose were determined by phenol-sulfuric acid method and HPLC.The ratio of peak area between mannose and glucose was worked out by HPLC. RESULTS The polysaccharide content of Dendrobium officinale distributed from 26.52% to 46.78%,the mannose content was between 13.36% and 25.25%,and the ratio of peak area between mannose and glucose was 2.48-5.91.CONCLUSION The polysaccharide contents of Dendrobii officinalis from different sources were diverse ,all of which accorded with the standard of Chinese Pharmacopoeia: The content of polysaccharide in Dendrobium officinale from Yunnan province exceeded that from Zhejiang province, and the mannose content in biennial samples were higher than annual samples.